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Characterize the effect of Ultrasmall Superparamagnetic Iron Oxide Nanoparticles (USPIOs) Uptake on Glioblastoma Multiforme (GBM) Biology

Rosita Tsz-Wai Pang, Department of Anatomy and Histology, Bosch Institute University of Sydney, NSW 2006, Australia; Steven S. Eamegdool, Department of Anatomy and Histology, Bosch Institute University of Sydney, NSW 2006, Australia; George Fatseas, Department of Anatomy and Histology, Bosch Institute University of Sydney, NSW 2006, Australia; Mark E. Koina, ACT Health, Canberra Hospital, ACT 2605, Australia; Binh T. T. Pham, School of Chemistry, Key Centre for Polymers and Colloids, University of Sydney, NSW 2006, Australia; Nguyen Pham, School of Chemistry, Key Centre for Polymers and Colloids, University of Sydney, NSW 2006, Australia; Brian S. Hawkett, School of Chemistry, Key Centre for Polymers and Colloids, University of Sydney, NSW 2006, Australia; Sam Adamson, Department of Anatomy and Histology, Bosch Institute University of Sydney, NSW 2006; Tailoi Chan-Ling, Department of Anatomy and Histology, Bosch Institute University of Sydney, NSW 2006


This study investigated the in vitro biological effects of USPIOs uptake on GBM function. USPIOs with a mean core diameter between 10 – 15 nm were loaded to CNS-1 cells cultures at different concentration (10-200µg/mL) and its biological effects were assessed in different time-point (6-24hr). Rat CNS-1 was chosen as our GBM model in this study because it was developed to obtain a histocompatible astrocytoma cell line with infiltrative and growth pattern similar to human gliomas. The uptake of USPIOs was analysed using the JEOL1011 transmission electron microscope (TEM) and the iron quantification was assessed using Graphite Furnace Atomic Absorption Spectrometry (GFAAS). The cell viability and the mitopotential were measured using the MUSE Count & Viability Kit and the MUSE Mitopotential Assay Kit. Bioenergetics was examined using Seahorse Mito Stress Test. TEM showed that USPIOs entered CNS-1 via clatherin coated pits which were then internalized in vacuoles. The biological effects of USPIOs on CNS-1 cell viability and mitopotential were dose and time-dependent. USPIOs at 5-200µg/mL decreased the cell viability of CNS-1 cells at 12 hr (Control: 100%±0, USPIO: 74.62%±2.11, P <0.05). USPIOs at 10-200µg/mL increased the percentage of total depolarized cells at 12 hr (Control: 0.03±0.01, USPIO: 0.37±0.12, P <0.05). Through these studies, it deepened our understanding of the biological effects of USPIOs on GBM function.

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