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Expression patterns of multiple antigens evaluated by immunochemistry in individual glioblastoma patient samples.

Michael E. Barish[5]*, Lihong Weng[1], Yubo Zhai[1], Massimo D’ Apuzzo[3], Behnam Badie[4], Stephen J. Forman[1], Christine E. Brown[1,2]*
Departments of [1]HematologyHCT and [2]Cancer Immunotherapy & Tumor Immunology, [3]Pathology, [4]Surgery PS-Neurosurgery, and [5]Neurosciences, City of Hope Beckman Research Institute and National Medical Center, Duarte, CA, 91010, USA.
* Corresponding authors


Cellular heterogeneity is a hallmark characteristic of ostensibly similar cell populations occurring during neural and glial development, and during tumorigenesis and tumor progression. For the latter case, large collections of formalin-fixed paraffin embedded (FFPE) clinical specimens are a valuable resource for evaluating gene expression across large study cohorts. Many of these patient samples consist of serial tumor sections each stained for a single antigen by chromogenic immunohistochemistry (IHC), as is standard clinical pathology practice. Quantifying multiple tumor related antigens in the context of GBM tissue is an essential step towards understanding the fundamental biology of these tumors as well as developing novel targeted therapies.

Towards this end, we have developed methods for combining semi-quantitative analysis of IHC processed tumor sections with regional annotation by a clinical pathologist. This has allowed us to consider patterns of antigen co-expression as well as spatial relationships with tumor structures and physiological status. We will present our observations on expression of tumor-associated antigens IL13Rα2, HER2 and EGFR, and associations with proliferation as indicated by Ki67 expression and the vasculature as marked by the endothelial cell marker CD34.

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