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Global expression analysis of microRNAs in Sox-2 positive glioblastoma stem-like cells

Jiri Sana, Central European Institute of Technology, Masaryk University, Brno, Czech Republic; Marek Vecera, Central European Institute of Technology, Masaryk University, Brno, Czech Republic; Petr Busek, Department of Biochemistry and Experimental Oncology, First Faculty of Medicine, Charles University, Prague, Czech Republic; Josef Srovnal, Institute of Molecular and Translational Medicine, Faculty of Medicine, Palacky University Olomouc, Olomouc, Czech Republic; Lenka Radova, Central European Institute of Technology, Masaryk University, Brno, Czech Republic; Pavel Fadrus, Department of Neurosurgery, Faculty Hospital Brno, Brno, Czech Republic; Aleksi Sedo, Department of Biochemistry and Experimental Oncology, First Faculty of Medicine, Charles University, Prague, Czech Republic; Ondrej Slaby, Central European Institute of Technology, Masaryk University, Brno, Czech Republic


Glioblastoma multiforme (GBM) is the most frequent malignant brain tumor characterized by very poor prognosis. Despite a treatment based on maximal surgical resection followed by concomitant chemoradiotherapy with temozolomide and adjuvant temozolomide, the median of overall survival is less than 15 months from diagnosis. This is caused by a high resistance to the therapy that is attributed to small subpopulation of glioblastoma stem-like cells (GSCs). Importantly, these cells express some pluripotent markers of healthy stem cells such as Sox-2. MicroRNAs (miRNAs) are small, non-coding RNAs that function as post-transcription regulators of gene expression. Therefore, they play also key role in maintaining of GSCs phenotype including a resistance to the chemoradiotherapy. In this study, our aim has been to identify miRNAs that are differentially expressed in Sox-2 positive GSCs and Sox-2 negative GBM cells. These miRNAs could play crucial role in maintenance of stem-like phenotype of GSCs as well as be prognostic and predictive biomarkers in GBM patients. To achieve our goal, we examined the global expression levels of ten paired Sox-2 positive serum-free cultivated cell lines and Sox-2 negative cell lines cultivated under fetal bovine serum condition. Individual cell lines have been derived from native GBM tissues obtained from patients, which underwent surgical resection. At this time, we analyze our data and the results will be presented on the conference.
This work was supported by grant NT13514-4/2012 of the Czech Ministry of Health, and by the project “CEITEC – Central European Institute of Technology” (CZ.1.05/1.1.00/02.0068).

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