List of communications


Novel in-vitro biomimetic microfluidic device to resemble the glioblastoma microenvironment

Jose M. Ayuso, Group of Structural Mechanics and Materials Modelling (GEMM). Centro Investigacion Biomedica en Red. Bioingenieria, biomateriales y nanomedicina (CIBER-BBN); Rosa Monge, Group of Structural Mechanics and Materials Modelling (GEMM). Centro Investigacion Biomedica en Red. Bioingenieria, biomateriales y nanomedicina (CIBER-BBN); Alicia Martínez-González, Institute of Applied Mathematics in Science and Engineering, Castilla-La Mancha Unversity, Spain ; Guillermo A. Llamazares, Group of Structural Mechanics and Materials Modelling (GEMM). Centro Investigacion Biomedica en Red. Bioingenieria, biomateriales y nanomedicina (CIBER-BBN); Javier Berganzo, MEMS/MST Department Ikerlan S. Coop., Mondragón, Spain; Aurelio Hernández-Laín, Multidisciplinar neuro oncology unit, Instituto investigación and Hospital Universitario 12 Octubre, Madrid, Spain; Jorge Santolaria, Department of Design and Manufacturing Engineering, University of Zaragoza, Spain; Manuel Doblaré, Group of Structural Mechanics and Materials Modelling (GEMM). Centro Investigacion Biomedica en Red. Bioingenieria, biomateriales y nanomedicina (CIBER-BBN); Pilar Sánchez-Gómez, Neuro-oncology unit. Health institute Carlos III-UFIEC, Spain; Víctor M. Pérez-García, Institute of Applied Mathematics in Science and Engineering, Castilla-La Mancha Unversity, Spain; Ignacio Ochoa, Group of Structural Mechanics and Materials Modelling (GEMM). Centro Investigacion Biomedica en Red. Bioingenieria, biomateriales y nanomedicina (CIBER-BBN); Luis J. Fernández, Group of Structural Mechanics and Materials Modelling (GEMM). Centro Investigacion Biomedica en Red. Bioingenieria, biomateriales y nanomedicina (CIBER-BBN);


Glioblastoma is one of the most infiltrating tumors. Characteristic hypercellular regions, named pseudopalisades, are observed in these tumors that have been explained as waves of migrating glioblastoma cells. These “waves” of cells are induced by oxygen and nutrient depletion caused by tumor-induced blood vessel occlusion. These structures seem to play an instrumental role on GBM spreading and invasion, however the recreation of these structures "in vitro" remains challenging. We present a new microfluidic model that mimics the dynamics of pseudopalisade formation. U-251 Cells have been embedded within a collagen hydrogel in a microfluidic device. Controlling the medium flow through the lateral microchannels we mimic the thrombotic event associated to this disease. Nutrient and oxygen starvation triggers a strong migratory process leading to pseudopalisade generation. The results validate the hypothesis of pseudopalisade formation, with an excellent agreement with a systems-biology model based on hypoxia-driven phenomenon. This interdisciplinary approach provides an example of the use of these microfluidic devices as advanced artificial systems with spatial gradients on nutrients that allow the validation of biological hypothesis.

Format: Oral communication

Organized by